纳米和微米一水草酸钙对Vero细胞毒性的浓度效应

采用扫描电子显微镜(SEM)、激光共聚焦显微镜(LSM)和流式细胞仪等技术比较了纳米级和微米级一水草酸钙(COM)对非洲绿猴肾上皮细胞(Vero)的毒性差异。结果显示,尺寸为100 nm和6μm的COM晶体都能引起Vero细胞活力下降、乳酸脱氢酶(LDH)释放量升高、透明质酸(HA)表达量增多、溶酶体完整性降低、线粒体膜电位下降、细胞死亡率升高和细胞周期滞留,表明这些晶体对Vero细胞都有损伤作用且呈现浓度依赖性,但纳米COM的毒性及其在细胞表面的粘附量均大于微米COM晶体。

Toxicity Difference of Nano and Micron Calcium Oxalate Monohydrate on Renal Epithelial Cells

Scanning electron microscope (SEM), laser confocal microscope (LSM), flow cytometry methods were used to comparatively study the toxicity difference of nano and micron calcium oxalate monohydrate (COM) on the African green monkey kidney epithelial (Vero) cells. The results revealed that both nano and micron COM could decrease cell viability, increase LDH release amount, up-regulate HA expression, decrease lysosomal integrity, decrease mitochondrial membrane potential, increased cell death rate and arrest cell cycle, which indicated that both nano and micron COM crystals could damage Vero cells and presented concentration dependent. But nano COM exhibited higher cytotoxicity and more adhesion amount than micron COM. This study may provide insights into the damage to renal epithelial cells induced by urinary crystals and the formation mechanism of kidney stones.