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稻壳纤维的柱层析性能研究
引用本文:钱俊青,郭爱芳.稻壳纤维的柱层析性能研究[J].功能高分子学报,2000,13(2):204-206.
作者姓名:钱俊青  郭爱芳
作者单位:浙江工业大学生物与环境工程学院,浙江,杭州,310014
摘    要:试验了稻壳纤维作为柱层析介质的性能,并制成亲和层析介质经脂肪酶。结果表明稻壳纤维层析过程为化学物理混合吸附过程,亲和层析性能良好,层析可将脂肪酶纯化2倍,回收率达81.6%。适合较大规模层析分离。

关 键 词:稻壳纤维  亲和层析  脂肪酶  层析介质  纯化

Study on Chromatography Property of Rice Hull Cellulose
Qian Jun-qing,Guo Ai-fang.Study on Chromatography Property of Rice Hull Cellulose[J].Journal of Functional Polymers,2000,13(2):204-206.
Authors:Qian Jun-qing  Guo Ai-fang
Abstract:The proporty of rice hull cellulose as chromatography is tested. The rice hull cellulose is memufacted to affinity chromatography medium to separate lipase. The results show chromatography process of rice hull cdlulose is a chemical and physical mixture adsorption. The property of affinity chromatography is well. The lipase can be purifyed to 2.11 times by chromatography, and the recovery ratio is 81.6 percent. Rice hull cellulose suits to apply larger scale chromatography separation.
Keywords:rice hull cellulose  affinity chromatography  lipase  
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