| 草鱼胰蛋白酶GT-A的紫外光谱及结构特性 |
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| 引用本文： | 张剑,刘忠义. 草鱼胰蛋白酶GT-A的紫外光谱及结构特性[J]. 光谱实验室, 2010, 27(3): 1098-1102 |
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| 作者姓名： | 张剑 刘忠义 |
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| 作者单位： | 湘潭大学生物与食品工程系,湖南省湘潭市羊古塘,411105;湘潭大学生物与食品工程系,湖南省湘潭市羊古塘,411105 |
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| 基金项目： | 湘潭大学博士启动课题 |
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| 摘 要： | 研究草鱼胰蛋白酶同工酶GT-A的结构特性,并探讨其结构特性与其性质之间的关系。用仪器分析方法,分析了草鱼肠道胰蛋白酶同工酶GT-A的紫外吸收特性以及圆二色谱(CD)。结果表明,GT-A在280nm处只有微弱的吸光度,在变性剂存在下,其紫外吸收大幅度增加,依据"双状态模型"计算得到其变性自由能为35.86KJ/mol。CD测定表明GT-A酶蛋白分子具有较少的α-螺旋、β-折叠以及β-转角等规则二级结构单元和较多的无规卷曲结构。这些信息结合起来可以合理解释GT-A的理化性质和动力学性质等。
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| 关 键 词： | 草鱼 胰蛋白酶 结构 圆二色谱 紫外吸收 |
Ultraviolet Spectra and Structural Properties of Trypsin Isozyme GT-A from the Grass Carp Intestines |
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| ZHANG Jian,LIU Zhong-Yi. Ultraviolet Spectra and Structural Properties of Trypsin Isozyme GT-A from the Grass Carp Intestines[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(3): 1098-1102 |
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| Authors: | ZHANG Jian LIU Zhong-Yi |
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| Affiliation: | (Department of Food and Biological Technolog,Xiangtan University,Xiangtan,Hunan 411105,P.R.China) |
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| Abstract: | Structural properties and properties of the grass carp （Ctenopharyngodon Idellus） intestine trypsin isoenzyme GT-A were investigated.The characteristic of ultraviolet absorption（UV） and circular dichroism （CD） scanning spectra were determined.The results showed that GT-A displayed a weak ultraviolet absorption at 280nm.In the present of denaturing reagent,its absorption significantly increased.According to ＂di-state model＂ ,the denaturation free energy was calculated to be 35.86 kJ/mol.The circular dichroism （CD） scanning spectra results indicated that GT-A contained small ratio of α-helix,β-sheet,and β-return but most random.According to these information,the physical-chemical and dynamical properties of GT-A can be legitimately explained and understood. |
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| Keywords: | Grass Carp Trypsin Structure Circular Dichroism UV Absorption |
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