表面活性剂在高效毛细管电泳中的作用

表面活性剂作为缓冲液添加剂已广泛用于高效毛细管电泳中，综述了阴离子、阳离子、两性离子、非离子及手性等多种表面活性剂在离子、中性分子、手性化合物、多肽和蛋白质分离等方面的作用，介绍了其作用机理与改善高效毛细管电泳分离的原理。

The Role of Surfactants in High Performance Capillary Electrophoresis(HPCE)

his paper reviews the role of ionic,non-ionic,and chiral surfactants as buffer additives in HPCE with38 references,Anionic surfactants, such as sodium dodecyl sulfate(SDS),form micelle above their car-boxymthyl celluloso(CMC)and the micell behaves as pseudo stationary phase in the micellar electrokineticchromatography(MEKC)separations of neutral , anionic and cationic analytes. Cationic surfactants could re-verse the direction of electroosmotic flow(EOF)and were employed in the capillary zone electrophoresis(CZE) determination of inorganic and organic anions. Surfactants of zwitterionb were used in the separationsof peptides and proteins to minimize the adsorptions of these biomolecules on the wall of capillary, or in theMEKC and/or CZE separations of peptides, Non-ionic surfactants,such as Triton X-100, might reduce orminimize EOF, while Tween 20 served as pseudo stationary phase in the MEKC separation of peptides.Mixed micelles, formed by SDS and chiral surfactants which are chemically synthesized or nattirally occurrringl might play an active role in the HPCE separations of enantiomers.