A comparative study of the immunogold labeling on H(2)O(2)-treated and heated epoxy sections. |
| |
| Authors: | S H Brorson A R Hansen H Z Nielsen I K Woxen |
| |
| Affiliation: | 1. Department of Pathology, Ullevål Hospital, Kirkeveien 166, 0407 Oslo, Norway;2. Bioingeniørutdanningen, Høgskolen i Oslo, Pilestredet 52, 0167 Oslo, Norway;1. Department of Cariology and Operative Dentistry, Tokyo Medical and Dental University, Tokyo, Japan;2. Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, People’s Republic of China;3. Department of Orthodontics and Pediatric Dentistry, Universidade Estadual Paulista-UNESP, Araraquara Dental School, Araraquara, Sao Paulo, Brazil;4. Department of Oral Biology, The Dental College of Georgia, Augusta University, Augusta, GA, USA;5. Department of Biomedical and Neuromotor Sciences DIBINEM, University of Bologna and IGM-CNR Unit of Bologna, Bologna, Italy;6. Department of Biomaterials, Faculty of Odontology, University of Toulouse III, Toulouse, France;7. Department of Materials Science Engineering, Georgia Institute of Technology, Atlanta, GA, USA;1. School of Mechanical Engineering, Jiangsu University, Zhenjiang, 212013, China;2. School of Energy and Power, Jiangsu University of Science and Technology, Zhenjiang, 212003, China;3. College of Electronics and Information Engineering, South-Central University for Nationalities, Wuhan, 430074, China;1. Inorganic Chemistry, Leipzig University, Johannisallee 29, 04103 Leipzig, Germany;2. Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France;1. Programa de Pós-Graduação em Sistemas e Processos Industriais, Universidade de Santa Cruz do Sul, Santa Cruz do Sul, RS 96815-900, Brazil;2. Departamento de Computação, e, Universidade de Santa Cruz do Sul, Santa Cruz do Sul, RS 96815-900, Brazil;3. Programa de Pós-Graduação em Tecnologia Ambiental, Universidade de Santa Cruz do Sul, Santa Cruz do Sul, RS 96815-900, Brazil;4. Departamento de Tecnologia e Ciência de Alimentos, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil;5. Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brazil |
| |
| Abstract: | The purpose of this study was to compare the intensity of the immunogold labeling of H(2)O(2)-treated and heated epoxy sections. Renal swine tissue with glomerular immune complex deposits with reactivity against IgG was embedded in epoxy resin. Immunogold labeling with anti-IgG was performed on sections from these blocks. Some of these sections were treated by H(2)O(2), others were heated in a citrate solution, while some were not treated at all. Some epoxy sections, which had been exposed to both H(2)O(2) and heat, were also exposed to the same immunolabeling. The heated epoxy sections obtained an yield of specific immunogold labeling, which was twice as large as the labeling of the H(2)O(2)-treated sections. The yield of immunolabeling of the sections that had been exposed to both H(2)O(2) and heat was not significantly different from the sections that were only exposed to heat. The non-treated sections were very weakly labeled with anti-IgG. We believe that both H(2)O(2) and heat have the ability to break some chemical bonds between the epoxy resin and the antigens, but heating in citrate buffer has a larger potential in this respect than H(2)O(2). We interpret the results from the combined treatment with H(2)O(2) and heat in the following way; the bonds that are broken by H(2)O(2) will also be broken by heating in citrate solution. The practical significance of these results is that heating in citrate buffer is a more convenient method for enhancing the immunolabeling of epoxy sections than treatment with H(2)O(2). |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
