Synthesis of Immobilized CMP-Sialic Acids and Their Enzymatic Transfer with Sialyltransferase |
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| Authors: | Yasuhiro Kajihara Takashi Ebata Hisashi Kodama |
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| Abstract: | A novel immobilization procedure for glycoproteins is based on the reacion of immobilized CMP-NeuAc (CMP-sialic acids) with sialyltransferase (see scheme). The transfer ability was tested under various conditions; a corresponding oligosaccharide or asialoglycoprotein acted as sialyl acceptor. The solid-phase synthesis of CMP-NeuAc is also described. |
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| Keywords: | Enzymes Glycoproteins Immobilization Sialic acids Solid-phase synthesis |
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