Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus |
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Authors: | L De Stefano I Rea E De Tommasi I Rendina L Rotiroti M Giocondo S Longobardi A Armenante and P Giardina |
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Institution: | 1.Unit of Naples-Institute for Microelectronics and Microsystems,National Council of Research,Naples,Italy;2.Dept. of Organic Chemistry and Biochemistry,University of Naples “Federico II”,Naples,Italy;3.LICRYL,INFM-CNR,Cosenza,Italy |
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Abstract: | A crystalline silicon surface can be made biocompatible and chemically stable by a self-assembled biofilm of proteins, the
hydrophobins (HFBs) purified from the fungus Pleurotus ostreatus. The protein-modified silicon surface shows an improvement in wettability and is suitable for immobilization of other proteins.
Two different proteins were successfully immobilized on the HFBs-coated chips: the bovine serum albumin and an enzyme, a laccase,
which retains its catalytic activity even when bound on the chip. Variable-angle spectroscopic ellipsometry (VASE), water
contact angle (WCA), and fluorescence measurements demonstrated that the proposed approach in silicon surface bioactivation
is a feasible strategy for the fabrication of a new class of hybrid devices. |
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