Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia |
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Authors: | JU RuiJun HUANG RenJie ZHOU Jia LI RuoJing ZHOU Peng ZHANG ZaoHua XIANG FeiJun XU DongJin LIU WeiXiang MA XingTian ZHANG Qiang & LU WanLiang State |
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Institution: | Key Laboratory of Natural and Biomimetic Drugs;School of Pharmaceutical Sciences,Peking University,Beijing 100191,China 2Department of Pharmacy,Fujian Health College,Fuzhou 350101,China 3China Academy of Chinese Medical Sciences,Beijing 100700,China 4Kangmei Pharmaceutical Co.,Ltd.,Puning 515300,China |
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Abstract: | The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient.Crude extract of salidroside was prepared by water-ethanol system,and purified by column chromatography of macroporous resins.Static adsorption and desorption studies were performed on six kinds of macroporous resins,and SP825 resin was chosen,followed by optimizing process parameters.The optimum sample volume,feed concentration,ratio of diameter to height,and feeding flow rate were 1.5 bed volumes(BV),15 mg/mL,1:10 and 1 BV/h,respectively.Dynamic desorption was performed consecutively with 8 BV of distilled water,3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h.After three cycles in separating 3.5 tons of rhodiola kirilowii roots,salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product.This study provides a novel method to separate salidroside for injectable use. |
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Keywords: | large-scale separation salidroside marcroporous resin rhodiola kirilowii injectable ingredient HPLC |
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