Spectroscopic Studies of α,γ-Disubstituted Trimethine Cyanine: New Fluorescent Dye for Nucleic Acids |
| |
| Authors: | I. V. Valyukh V. B. Kovalska Y. L. Slominskii S. M. Yarmoluk |
| |
| Affiliation: | (1) Physical Department, Kyiv Taras Shevchenko National University, 64 Volodymyrs'ka St., 03033 Kyiv, Ukraine;(2) Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine;(3) Institute of Organic Chemistry, National Academy of Sciences of Ukraine, 5 Murmanska St., 02094 Kyiv, Ukraine |
| |
| Abstract: | Spectral properties of 3-methyl-2-3-[3-methyl-1,3-benzothiazolo-2(3H)-ylidene]-1,4-cylopentadien-1-yl-1,3-benzothiazolo-3-ium tosylate (Cyan-Cpentd) in a free state and in the complexes with nucleic acids and synthetic polynucleotides have been investigated by absorption and fluorescence spectroscopy. Significant fluorescence intensity enhancement of dye-nucleic acids complexes is observed. For the first time Cyan-Cpentd is proposed as a new probe for nucleic acid detection. Binding mechanism of Cyan-Cpentd is discussed in view of the NA-ligand interaction models. |
| |
| Keywords: | Nucleic Acid Fluorescent Detection Cyanine Dyes |
| 本文献已被 SpringerLink 等数据库收录！ |
|
