Semi-xylenol orange and its purification by high-pressure liquid chromatography |
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| Authors: | Smedes F Decnop-Weever L G Uyen N T Nieman J Kragten J |
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| Institution: | Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, Amsterdam, The Netherlands. |
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| Abstract: | By Mannich condensation of o-Cresol Red, iminodiacetic acid and formaldehyde, Semi-Xylenol Orange (SXO) has been prepared in a 10-hr batch-procedure with a yield of about 30%. From the crude product SXO has been isolated by reversed-phase HPLC with perchloric acid-acetone mixtures as the mobile phase and C(18)-bonded silica as the stationary phase. The SXO fraction was freed from accompanying perchloric acid by a second separation on the same column, with water as eluent. After elution with acetone, the SXO was crystallized by evaporation. |
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