Peroxidase-Like Platinum Clusters Synthesized by Ganoderma lucidum Polysaccharide for Sensitively Colorimetric Detection of Dopamine |
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| Authors: | Xiang Lai Yu Han Jie Zhang Jinyu Zhang Weifeng Lin Zhiwei Liu Longgang Wang |
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| Affiliation: | 1.Key Laboratory of Applied Chemistry, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China; (X.L.); (Y.H.); (J.Z.); (J.Z.); (Z.L.);2.Department of Molecular Chemistry and Materials Science, Weizmann Institute of Science, Rehovot 76100, Israel; |
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| Abstract: | The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA using Ganoderma lucidum polysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3’,5,5’-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1–100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field. |
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| Keywords: | dopamine detection platinum colorimetric Ganoderma lucidum polysaccharide |
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