| 刚果红-孔雀石绿-左氧氟沙星分光光度法测定左氧氟沙星的含量 |
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| 引用本文： | 胡卫平,王鑫,董学芝,张蕾,焦嫚.刚果红-孔雀石绿-左氧氟沙星分光光度法测定左氧氟沙星的含量[J].分析科学学报,2012,28(2):257-260. |
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| 作者姓名： | 胡卫平 王鑫 董学芝 张蕾 焦嫚 |
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| 作者单位： | 河南大学化学化工学院环境与分析科学研究所,河南开封,475004 |
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| 基金项目： | 国家自然科学基金，省部共建河南大学科研项目 |
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| 摘 要： | 在pH=3.55的NaAc-HAc缓冲溶液中,左氧氟沙星对刚果红(CR)和孔雀石绿(MG)反应生成的产物在可见光区有明显的作用,在618 nm波长处测定其吸收强度,结果表明体系的吸光度与左氧氟沙星的浓度在2～60μg.mL-1范围内呈现良好的线性关系,线性相关系数R=0.9905,检出限为0.053μg.mL-1。该方法可用于盐酸左氧氟沙星片剂、胶囊以及尿液中左氧氟沙星含量的测定。
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| 关 键 词： | 左氧氟沙星 刚果红 孔雀石绿 分光光度法 |
Determination of Levofloxation with Congo Red-Malachite Green-Levofloxacin System by Spectrophotometry |
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| HU Wei-ping
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WANG Xin
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DONG Xue-zhi
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ZHANG Lei
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JIAO Man.Determination of Levofloxation with Congo Red-Malachite Green-Levofloxacin System by Spectrophotometry[J].Journal of Analytical Science,2012,28(2):257-260. |
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| Authors: | HU Wei-ping WANG Xin DONG Xue-zhi ZHANG Lei JIAO Man |
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| Institution: | Man(Institute of Environmental and Analytical Sciences,College of Chemistry and Chemical Engineering, Henan University,Kaifeng 475004) |
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| Abstract: | In a NaAc-HAc buffer solution(pH=3.55),the absorbance of the chelate complex between congo red(CR) and malachite green(MG) was enhanced by levofloxacin(LVFX),and a method for the determination of levofloxacin was therefore developed by spectrophotometric measurement at 618 nm.A good linearity in the range of 2~60 μg/mL and a detection limit of 0.053 μg/mL were obtained.The method has been applied to the determination of levofloxacin in tablets,capsules and urine samples and compared to the pharmacopoeia method with satisfactory results. |
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| Keywords: | Levofloxacin Congo red Malachite green Spectrophotometry |
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