| 高效液相色谱-串联质谱法测定稻米和稻壳中井冈霉素A的残留 |
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| 引用本文： | 关文碧,王晨,张红艳.高效液相色谱-串联质谱法测定稻米和稻壳中井冈霉素A的残留[J].分析科学学报,2012,28(1):136-138. |
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| 作者姓名： | 关文碧 王晨 张红艳 |
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| 作者单位： | 中国农业大学理学院,北京,100193 |
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| 摘 要： | 本文建立了稻米和稻壳中井冈霉素A的高效液相色谱-串联质谱(HPLC-MS/MS)分析方法。样品用甲醇-水(9+1)涡旋提取,过滤膜后进行HPLC-MS/MS分析。用多反应监测技术确定井冈霉素A的两对离子对m/z498.2/178.1、m/z498.2/336.1为定性离子对,m/z498.2/178.1为定量离子对。方法的线性范围为0.005～0.2mg/L,其中稻壳的线性相关系数为0.9993,稻米的线性相关系数为0.9988。稻米、稻壳的0.05、0.1、0.5 mg/kg三个浓度的添加回收率为71.6%～88.8%,相对标准偏差(RSD)为1.89%～8.16%。方法的定量限(LOQ)为5μg/kg。
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| 关 键 词： | 井冈霉素A 高效液相色谱-串联质谱法 残留分析 稻米 稻壳 |
Determination of Validamycin A Residues in Rice and Rice Hull by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| GUAN Wen-bi
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WANG Chen
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ZHANG Hong-yan.Determination of Validamycin A Residues in Rice and Rice Hull by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Analytical Science,2012,28(1):136-138. |
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| Authors: | GUAN Wen-bi WANG Chen ZHANG Hong-yan |
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| Institution: | (College of Science,China Agricultural University,Beijing 100193) |
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| Abstract: | A method for the determination of validamycin A residues in rice and rice hull was developed using high performance liquid chromatography-tandedm mass spectrometry(HPLC-MS/MS).Validamycin A was vortex extracted with methanol-water(9+1,V/V) and then analyzed by HPLC-MS/MS.For the samples,the recoveries ranged from 71.6% to 88.8% and the relative standard deviations(RSDs) ranged from 1.9% to 8.2% were obtained.The limit of quantification(LOQ) was 5 μg/kg with a linear range of 0.005 to 0.2 mg/L. |
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| Keywords: | Validamycin A residue High performance liquid chromatography-tandem mass spectrometry Rice Rice hull |
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