烯唑醇分子印迹固相萃取介质的制备与应用

以烯唑醇为模板分子,采用可逆加成断裂链转移沉淀聚合法制备了分子印迹聚合物,对该聚合物的吸附性能进行了研究,结果表明,该印迹聚合物的最大吸附量为39.3mg/g,非印迹聚合物的最大吸附量为14.5mg/g;并通过电镜分析对印迹聚合物的形貌进行表征,结果表明,印迹聚合物微球粒径均一,分散性好。将其作为固相萃取填料,结合高效液相色谱方法,对苹果中残留烯唑醇进行分析。在优化的条件下,烯唑醇检出限为1.64×10-3 mg/kg;回收率为93.2%~100.3%;该柱与常用的C18柱相比,富集与净化效果更好。

Preparation and Application of Diniconazole Molecularly Imprinted Solid Phase Extraction

Molecularly imprinted polymer was prepared by reversible addition fragmentation chain transfer polymerization with diniconol as template molecule. The results showed that the maximum adsorption capacity of the imprinted polymer was 39.3mg/g, the maximum adsorption capacity of non-imprinted polymers is 14.5mg/g.The adsorption performance was studied and its morphology was characterized by electron microscopy. The results showed that Imprinted Polymer uniform particle size and good dispersibility. It was used as a solid phase extraction filler and combined with high performance liquid chromatography to analyze the residual diniconazole in apples. Under optimized conditions, the detection limit of diniconazole was 1.64×10-3 mg/kg;the recovery was 93.2~100.3%.The column could enrich and purify the diniconazole with better effect than the common C18 column.