一种具有体外和体内生物活性氨基酸聚合物的制备和表征

以6-氨基己酸为主链,通过熔融缩聚与其它五种氨基酸制备了一种六元氨基酸聚合物,对该聚合物的理化性能进行表征,并评价其作为骨组织修复材料的体外和体内生物活性,为骨组织修复材料研究提供思路。通过傅里叶转换红外光谱分析(FT-IR)、X射线衍射分析(XRD)、差示扫描量热分析(DSC)和热重分析(TG)对合成的聚合物进行表征。将聚合物浸泡于模拟体液(SBF)中,5天后用扫描电子显微镜(SEM)观察其体外生物活性;将聚合物植入新西兰大白兔肌肉和股骨髁骨部位,8周后观察聚合物体内生物活性。结果表明,合成的聚合物为酰胺键结构,熔融温度为205.9℃,热结晶温度为159.5℃。合成的聚合物具有良好的体外生物活性,聚合物在肌肉和股骨髁骨中无炎症反应,与肌肉和骨组织直接接触,无新生纤维组织包裹,在股骨髁骨中具有较好的骨传导性能。

The Preparation and Characterization of Amino Acids Polymer with in vitro and in vivo Bioactivity

A polymer mainly prepared with 6-aminohexanoic acid and another assistant five kinds of amino acids were synthesized in this study for evaluating the physicochemical characteristic, in vitro and in vivo bioactivity as bone repair materials. The transforms infrared (FT-IR)、X-ray diffraction analysis (XRD)、differential scanning calorimetry(DSC) and thermal gravity analysis(TG) were investigated and characterized;The scanning electron microscopy (SEM) was tested for evaluating the in vitro bioactivity after soaking the polymer into simulated body fluid (SBF) with five days;The implantation of polymer into muscles and femur condyle of rabbits was assessed for evaluating the in vivo bioactivity of the polymer. The results showed that the polymer possessed amide band and was semi-crystallization structure, further the melting temperature and crystallization temperature was 205.9℃ and 159.5℃, respectively;The polymer could induce the form of apatite in the surface of polymer, showing good bioactivity;The implantation of polymer into muscles and femur condyle of rabbits results stated that the polymer had no visible inflammation and had contacted directly between implants and tissue without newly formed fibrous connective tissue. The fluorescence results showed that the polymer may possessed ectopic osteogenesis and have exactly great osteoconductivity in vivo.