| Abstract: | ABSTRACT The possibility of using the iodide method and the method of bleaching of 4-nitroso-N, N'-dimethylaniline, developed originally for homogeneous aqueous medium for the determination of singlet oxygen production, was tested for water-insoluble nonpolar senzitizers adsorbed on the silica gel support. Sensitizers of the porphyrin and metalloporphyrin type (tetraphenylporphin (TPP), protoporphyrin IX (PPIX), tetra(4-nitrophenyl)porphin (TPP(NO2)4 and complexes of TPP with some divalent and trivalent metals, Cd(II)TPP, Zn(II)TPP, Co(II)TPP, Cu(II)TPP, Ni(II)TPP, Mn(II)TPPCI, and Fe(II)TPPCl) were used as model compounds. It was found that both methods used gave similar values of 1O2. The relative 1O2 production of the compounds studied decreases in the order PP=Zn(II)TPP=Cd(II)TPP > TPP(NO2)4> PPIX> nonactive substances. The cytotoxic effect of 1O2 Produced by these substances on the silica gel support was tested using E. coli DHS-alfa bacteria. The relative 1O2 production values correlate with the antibacterial activity of the adsorbed substances exposed to light. |
