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Internal Standard Quantitative Method Of Tetrodotoxin By Capillary Electrophoresis
Abstract:ABSTRACT

An internal standard quantitative method of tetrodotoxin by capillary electrophoresis was developed, ephedrine hydrochloride being chosen as the internal standard of tetrodotoxin. The calibration curve was built with the correlation coefficient of 0.9997, and the linear range was from 0.01 mg.ml?1 to 10.0 mg-ml?1 with the detection limit of 1.0 x 10?3 mg.m1?1. RSD of the migration time of ephedrine hydrochloride and tetrodotoxin were all less than 1.0 %. The method was applied to determine tetrodotoxin in the poisonous frilled fish samples.
Keywords:Internal standard quantitative method  Capillary electrophoresis  Tetrotodoxin  Ephedrine hydrochloride
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