| Abstract: | Abstract The fluorescent reaction between ascorbic acid(AA) and 2-cyanoacetamide was studied. The experimental results showed that AA can react with 2-cyanoacetamide at pH 12.9–13.3, and form a fluorescent product, which emitted strong fluorescence. The fluorescence intensity was measured at excitation and emission wavelengths of 329 and 380nm, respectively. A linear relationship was obtained between the fluorescence intensity and AA concentration in the range of 0.1–50μg/ml, the regression coefficient is 0.9994, and the detection limit of AA is 0.03μg/ml. (signal-to-noise=3). |
