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Photochehical Fluorimetric Determination of Primaquine in a Flowing Solvent with Application to Blood Serum
Abstract:Abstract

Primaquine in a flowing solvent is photolyzed with a 200 W Xe-Hg arc lamp and the intensely fluorescent photochemical product is measured in a fluorimeter. The effect of solvent composition and pH, irradiation time, and excitation conditions are evaluated. Primaquine in blood serum is measured by introducing a sample of blood serum into the flowing stream and performing the photochemical-fluorescence measurement. The fluorescence background of blood serum was very low allowing a detection limit of less than 0.1 μg/mL. Excellent recoveries were obtained.
Keywords:Fluorescence  photochemical conversion  blood serum  primaquine.
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