| Abstract: | A new method for phytochelatins by high-performance liquid chromatography (HPLC) was developed based on a condensation reaction with monobromobimane to produce fluorescent derivatives. Glutathione, H-(γ-glutamic acid-cysteine)2-glycine-OH, H-(γ-glutamic acid-cysteine)3-glycine-OH, H-(γ-glutamic acid-cysteine)4-glycine-OH, H-(γ-glutamic acid-cysteine)5-glycine-OH, and H-(γ-glutamic acid-cysteine)6-glycine-OH were well separated, with retention times between 14.68 and 22.0 min. The HPLC method had good linearity (r < 0.9991) between 0.1 mg L?1 and 100 mg L?1. The limits of quantification for the analytes (S/N = 3) were 0.08, 0.3, 0.05, 0.3, 0.5, and 0.8 mg L?1, respectively. The recoveries were between 83.0% and 101.33% with relative standard deviations less than 2%. The reported method is simple, accurate, and suitable for the determination of phytochelatins. |
