| Abstract: | Abstract Charge transfer reaction is described for the assay of certain cardiovascular drugs; carbochromen hydrochloride, verapamil hydrochloride, acebutolol hydrochloride, carazolol and propranolol hydrochloride. the three acceptors, p-chloranilic acid (p-CA), dichlorophenyl-indophenol (DCPIP) and 2,3-dichloro 5,6-dicyano p-benzoquinone (DDQ) have been used to carry out such assay. the reaction is based on the proton transfer from the p-CA to the drug base or electron transfer from the drug base to either DCPIP or DDQ and the subsequent possible formation of resonance hybrids of charge transfer complexes. Optimization of the reaction conditions has been investigated. Obediance to Beer's law permitted the assay of these drugs in their dosage form. Calculating log ε for different chromogens, the method sensitivity is decreasing in order of using DCPIP, DDQ & p-CA. the method has been compared with the traditional U.V. absorbance spectrophotometric method and found to be of equal accuracy (t-test) and equal reproducibility)(F-test). |
