| Abstract: | Abstract Allopurinol and uric acid are successfully assayed either in laboratory made mixtures or in urine using derivative spectrophotometry. The former is determined through first - derivative signal (1 D) measurement at 284 nm, and the latter through second - derivative signal (2D) measurement at 293 nm. The colinearity of these values versus concentration of both compounds in the range 0.2–1.2 mg% permits their determination with high accuracy and good reproducibility. The method is recommended to monitor the allopurinol therapy in treatment of chronic gouty patients. |
