| Abstract: | Abstract It was found that gmanylic acid (GMP) can be selectively completed with Tb3+ at pH 6.0-6.6, which then emits strong fluorescence characteristic of Tb3+. This reaction can be used for the determination of GMP in presence of adenylic acid (AMP), uridylic acid (UMP) and cylidylic acid (CMP). A linear relationship is obtained between the fluorescence intensity and GMP concentration in the range of 2.0×10?7 - 1.0×10?4M. The detection limit is 2.0×10?8 M. The results showed that the composition ratio and apparent stability constant of GMP-Tb complex were 1:1 for GMP Tb3+- and 2.3×10?5, respectively. |
