Determination of Melphalan and Chlorambucil in Tablet Dosage form Using High-Performance Liquid Chromatography and Amperometric Detection

Abstract

We developed a liquid chromatographic method for the measurement of melphalan and chlorambucil in tablets, using a reversed phase C18 column with isocratic elution and amperometric detection. For melphalan the potential was set at +0.95V and chlorambucil at + 0.90V. The method is simple, sensitive and may also be applicable to pharmacokinetic studies.