Simultaneous Quantification of Ceftazidime and Pyridine,its Main Degradation Product,by High-Performance Liquid Chromatography

ABSTRACT

A high performance liquid chromatographic method for the simultaneous assay of ceftazidime and pyridine, the principal degradation product of ceftazidime, is described. The method was fully validated in terms of recovery, linearity, selectivity and precision. An application was done on stability of ceftazidime at 40 mg.mL¹ in infusion solutions 0.9% NaCl and 5% glucose on 24 hours in ambulatory infusion device. Quantification results of pyridine were more linear and accurate than ceftazidime. Pyridine was a good label in ceftazidime stability studies.