| Abstract: | Abstract Based on the immunoreaction equation and the quality function law, a method of determining the binding ratio and the affinity constant of antibody/antigen immunoreaction has been developed by measuring resonance light scattering (RLS) signals of the polystyrene latex particles. According to the method, the binding ratio and affinity constant of HIgG binding with goat anti-HIgG equal 0.71 and 47.3 mL/mg; that is, 1 mol HIgG can be bound with 1.4 mol goat anti-HigG. This new method is much simpler and easier to carry out when studying immunoreaction kinetics and determining the binding ratio and affinity constant. |
