A Simplified Method for Determination of Nifedipine in Human Plasma by High Performance Liquid Chromatography

Abstract

A simplified high performance liquid chromatographic method was developed to determine the levels of nifedipine in human plasma. Nifedipine and the internal standard, 11-ketoprogesterone, are extracted from alkalinized plasma into organic solvent. The organic solvent is then evaporated to total dryness and the analytes are reconstituted with mobile phase and chromatographed. The limit of quantitation is 10 ng/ml using a 1.0 ml plasma sample.