| Abstract: | A portable immuno-microchip system consisting of low-cost biocompatible-polystyrene chips, a mini-vacuum pump, and a base unit for a LED light source as a miniaturized optical readout was devised. The surfaces of the micro-channels were processed with plasma to be more hydrophilic and were characterized by kinetic coating with a CCD scanning method. To demonstrate the feasibility of this novel system for the determination of a small molecule control assay, a simple, specific, and rapid method was established for drug analysis of morphine and its analogs, 6-monoacetylmorphine and morphine-3-glucuronide. Morphine-3-site antigen and a multi-target polyclonal antibody to morphine and derivatives were prepared and a competitive immunoassay was performed on the chip. The measurement can be carried out automatically and avoids time-consuming incubation steps. A detection sensitivity of below 1.0 ng/mL was achieved, which is comparable with the enzyme-linked immunosorbent assay (ELISA) technique. |
