| Abstract: | Abstract 2-(Diphenylacetyl)-1,3-indanedione-1-hydrazone and its derivatives where the NH2 has been replaced by a substituted NH or an imino group give fluoresence enhancement with trichothecene mycotoxins. Absolute amounts of T-2 toxin as low as 50 ng are detectable. Detection limits for HT-2, Diacetoxyscirpenol, Neosolaniol, and Fusarenon-X range from 0.1 to 4μg. The advantage in using 2-(diphenylacetyl)-1,3-indanedione-1-hydrazone (DIPAIN) derivatives for the detection of trichothecene mycotoxins is that response time is based on the rate of formation of a molecular association complex between the toxin and the detector reagent rather than on the chemical reactivity of the toxin. Hence, sensitive detection or mycotoxins can be achieved simply and rapidly at 25°C. Since DlPAlN derivatives have been found to be direct-acting reagents capable of detecting droplets of dissolved trichothecenes, it is speculated that they may be useful as coatings in optical waveguide devices or in other devices that are developed as field detectors for aerosols that contain trichothecene mycotoxins. The DlPAlN reagents may also be used in kits that are designed to detect surface contamination by trichothecene mycotoxins. |
