Fluorometric Method for Determination of Transketolase Activity

Abstract

A new method for rapid and accurate determination of transketolase is studied. The reaction is monitored by coupling the system with the glyceraldehyde-3-phosphate dehydrogenase system and flurometrically measureing the production of NADH. The assay is rapid and convenient. It responds linearly to transketolase activity as low as 0.1 units in three ml of reaction mixture.