| Abstract: | Abstract A detailed study of the influence of the eluent composition, such as the ratio of the concentration of water to acetonitrile and the apparent pH, for the analysis of nonapeptides by high-performance liquid chromatography is described. Selection of the optimum conditions is based on maximizing the separation of the minor components from the major component in a minimum elution time. This investigation also describes the effect of an elution gradient, the wavelength setting for the absorbance detection, recycling the main component through the column and using microparticulate octadecyl columns from different manufacturers. |
