Direct HPLC Separation of Carazolol Enantiomers on a Cellulose Tris-3, 5-Dimethylphenyl Carbamate Column

Abstract

Direct liquid chromatographic resolution and optimization of carazolol are accomplished without any derivatizations on a cellulose tris-3, 5-dimethylphenyl carbamate column (known as OD-CHIRALCEL). Maximum stereochemical resolution (R) of 2.2 is obtained. The effects of 2-propanol and diethylamine content in mobile phase and effect of temperature on capacity factor (k), stereochemical separation factor (α) and stereochemical resolution factor (R) is demonstrated.