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Separation and Determination of Low Abundant Flavonoids in Scutellaria Baicalensis Georgi by Micellar Electrokinetic Capillary Electrophoresis
Abstract:Abstract

Flavonoids are secondary metabolites in many plants with diverse biological activities. A simple and rapid micellar electrokinetic capillary electrophoresis method was developed for simultaneous determination of three low abundant flavonoids (chrysin, 5,7,4′-trihydroxy-8-methoxyflavone, and skullcapflavone II) in Scutellaria baicalensis Georgi within 12 min. The calibration curves revealed a good linear relationship between the peak areas of analytes and their concentrations (11.38 to 436.67 mg/L). The standard deviations of the migration time of the three flavonoids were determined to be 0.64%, 0.83%, and 1.17%, and peak areas were 6.14%, 7.57%, and 8.33%. Recoveries ranged between 90.37 and 109.4%.
Keywords:8-hydroxymethylflavone  chrysin  Micellar electrokinetic capillary electrophoresis  oroxylin-A  skullcapflavone II  wogonin
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