A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

Abstract

Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.