Abstract: | Abstract It was found that ATP could form a ternary complex with Tb3+ and Phen, which can emit the characteristic fluorescence of Tb3+. The experiment showed that the fluorescence intensity of the system was maxmium in the following conditions: pH of the solution is 7.2, the concentrations of Tb3+ and phenanthroline (Phen) are 2.0×10-5mol.L-1 and 2.0 × 10-4mol.L-1, respectively. At the optimum conditions, a linear relationship is obtained between the fluorescence intensity and ATP concentration in the range of 1.0×10-6—1.0×10-5mol.L-1. Traces of ATP in drugs (ATP disodium salt tablets) can be determined using the standard addition method. The luminescence mechanism of the system was discussed. |