| Abstract: | Abstract S-adenosylmethionine plays an important role in many biochemical reactions, including human health maintenance and disease prevention. This paper proposes a simplified, rapid, and easy high performance liquid chromatography (HPLC) with fluorimetric detection method of S-adenosylmethionine and its product, S-adenosylhomocysteine. Determination of S-adenosylmethionine and S-adenosylhomocysteine was by HPLC with fluorimetric detection by the formation of the fluorescent 1,N 6-ethanoderivatives of these compounds, which showed a linear concentration range of 310?6–310?8 molL?1 for S-adenosylmethionine and 510?7–510?9for S-adenosylhomocysteine; the detection limits of the method ranged in the nanomolar domain. The method applied to real plasma samples lead to values of 60.1±19.6 for S-adenosylmethionine and 24±14.1 nmolL?1 for S-adenosylhomocysteine. |
