Gas Chromatographic Determination of Microgram and Nanogram Quantities of Histamine as Its Na-Trifluoroacetyl-Nim-Carbethoxy Derivative

Abstract

A method has been devised for the quantitative estimation of microgram and nanogram quantities of histamine as its N^a-trifluoroacetyl-N^im-carbethoxy derivative. Trifluoroacetic anhydride was used for the formation of N^a-trifluoroacetyl derivative and ethoxyformic anhydride was used for the N^im-carbethoxy derivative. Quantitative determinations were made in the range of 20 to 100 micrograms and 250 and 1000 nanograms with a mean standard deviation of 2–3%. The standard curves were linear.