Abstract: | ABSTRACT The solid surface fluorimetry (SSF) for the determination of 6-thioguanine (6-TG) has been presented. Several experimental conditions such as the type of solid substrate, pH of the medium, rigidity state of substrate, drying condition etc. were optimized. The new and highly sensitive procedure has a linear range of 3.3~668.6ng/spot with the correlation coefficient 0.999, the relative standard deviation (RSD) 3.16% and the limit of detection 0.47 ng/spot corresponding to three times the standard deviation of the blank (n=11). The recovery of standard 6-TG added to urine was over the range of 97.4-107.4%. The method is simple, rapid and sensitive and can be applied to analysis of urine without interference. |