A Simple Simultaneous Colorimetric Determination of Primary and Secondary Amines with Fluorescamine

Abstract

Primary and secondary amines react reproducibly with fluorescamine to form pyrrolinones or aminoenone type chromophores with long wavelength absorption maxima in the 375–410 and 310–320 nm regions, respectively. A simple procedure has been worked out for the simultaneous colorimetric determination of primary and secondary amines.