| Abstract: | Abstract A wireless remote‐query disposable magnetoelastic (ME) biosensor was developed for the assay of acid phosphatase (ACP). The sensor was fabricated by applying a magnetoelastic ribbon with a layer of pH‐sensitive polymer and upon it a sensing film containing bovine serum albumin (BSA) and adenosine‐5′‐monophosphate (5′‐AMP). The ACP‐catalyzed hydrolysis of 5′‐AMP decreases the solution pH, resulting in the polymer shrinking and consequently the resonance frequency of the magnetoelastic sensor increasing. The kinetic parameters were measured to be 1.64×10?3 M (Michaelis constant) and 130 Hz/min (maximum initial rate). The proposed sensor can determine 0.2 to 1.2 U/ml of ACP. |
