| Abstract: | Abstract An enzyme immunoassay for resin acids was developed using polyclonal antibodies raised in rabbits. Dehydroabietic acid (DHA) was chosen as a representative resin acid due to its common occurrence and relatively high concentration and toxicity in pulp effluents. Dehydroabietylamine was conjugated to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA), respectively. The former was used as the immunogen and the latter as the coating antigen. By indirect ELISA, using the biotin-streptavidin system, antiserum can be diluted to 1:30,000 when the coating antigen was used at a concentration of 25 ng/100μl/well. The 50% inhibition concentration (IC50) and the detection limit were determined as 20.2 ppb and 1.9 ppb, respectively. |
