| Abstract: | Abstract A new spectrofluorimetric method was developed for the determination of trace amounts of lecithin. Using enoxacine (ENX)‐terbium ion (Tb3+) as a fluorescent probe, in a buffer solution at pH=5.80, lecithin can remarkably reduce the fluorescence intensity of the ENX‐Tb3+ complex at λ=545 nm; the reduced fluorescence intensity of the Tb3+ ion is proportional to the concentration of lecithin. Optimum conditions for the determination of lecithin were also investigated. The linear range and detection limit for the determination of lecithin were 1.96×10?7–9.8×10?6 mol l?1 and 9.74×10?8 mol l?1. This method is simple, practical and relatively free of interference from coexisting substances, and can be successfully applied to assess lecithin in serum samples. |
