Quantitation of Chloroquine Phosphate And Primaquine Phosphate In Tablets Using High-performance Liquid Chromatography

Abstract

Reverse phase high-performance liquid chromatography methods for the quantitation of chloroquine phosphate and primaquine phosphate in tablets have been developed. The methods require one column and 2 mobile phases for complete analysis of both drugs when present in combination. The methods are accurate and precise with percent relative standard deviations based on 6 injections of 0.5 and 0.9 for chloroquine and primaquine, respectively. The results of single ingredient, chloroquine phosphate tablets are in excellent agreement with the USP-NF method which is nonspecific. Primaquine interfers with the USP-NF method and not with the developed HPLC method. Both drugs appear to be very stable to heat.