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Flow-Injection Determination of Glucose by a Photoinduced Chemiluminescent Reaction
Abstract:Abstract

A flow-injection procedure for the photochemical determination of glucose has been developed. The method is based on the photo-oxidation of glucose sensitized by 9,10-anthraquinone-2.6-disulfonate (disodium salt). The hydrogen peroxide formed in the photochemical reaction was measured by means of the chemiluminescent reaction with luminol and hematin. A linear calibration graph was obtained over the range 2.0x10?6-8.5 x 10?5 mol L?1. The method was applied to determining glucose in blood serum, urine and fruit juices.
Keywords:Glucose  flow-injection  photochemical reaction  chemiluminescent detection  biological fluids
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