Fluorometric Determination of Cellulase

Abstract

A fluorometric procedure is described for the determination of the enzyme cellulase. The method is based upon the hydrolysis of the nonfluorescent substrate, resorufin acetate, by the enzyme to give the highly fluorescent resorufin (λ_ex = 540 mμ;, λ_em = 580 mμ). By this procedure from 0.00010 to 0.060 units per ml. of cellulase can be determined with an accuracy and deviation of about 1.5%. Evidence is offered to demonstrate cellulase, and not esterase, activity.