Microbial Membrane Electrode for Steroid Assay

Abstract

Polyacrylamide - immobilized Nocardia opaca was coupled to a rotating glassy carbon electrode to form a microbial membrane electrode for the estimation of steroid hormones. The principle of assay is baaed on the detection of reduced artificial electron acceptor. The concentration dependence for 3 keto- 4- enesteroids solubilized in methanol was demonstrated.