A Validated Voltammetric Procedure for Quantification of the Antifungal Drug Griseofulvin in Bulk Form,Tablets, and Biological Fluids at a Mercury Electrode |
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Abstract: | Abstract Griseofulvin is an antifungal antibiotic used to treat various pathogenic mycotic diseases. The voltammetric behavior of griseofulvin at a hanging mercury drop electrode in Britton‐Robinson buffers of pH 2–11.5 was studied and discussed. A fully validated sensitive square‐wave adsorptive cathodic stripping voltammetric procedure was described for direct determination of bulk griseofulvin substance. The procedure was based on the reduction of the >C?O double bond of griseofulvin molecule following its preconcentration onto a hanging mercury drop electrode in a Britton‐Robinson buffer of pH 10. Limits of detection (LOD) and quantitation (LOQ) of 5.8×10?10 M and 1.93×10?9 M bulk griseofulvin were achieved, respectively. The proposed stripping voltammetric procedure was successfully applied to assay griseofulvin in tablets and in spiked human serum and urine samples. LOD of 8.65×10?10 M and 6.6×10?9 M and LOQ of 2.88×10?9 M and 2.2×10?8 M griseofulvin in spiked human serum and urine samples, respectively, were achieved. |
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Keywords: | Griseofulvin ultrafulvin cyclic voltammetry square‐wave adsorptive stripping voltammetry |
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