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Organic-Phase Enzyme Electrode for the Determination of Phenols in Olive Oils
Abstract:Abstract

A rapid procedure for determining phenols in olive oils, based on an organic-phase enzyme electrode, is described. Direct assays are performed in chloroform solutions which support the tyrosinase activity. This class (phenol)-specific enzyme strongly adheres to the surface of the graphite transducer. The resulting wall-jet detector offers effective flow injection operation, with a detection limit of 4×10?7 M (0.8 ng) phenol and sample frequency of 60 h?1. Applicability to olive-oil samples of different origins is illustrated.
Keywords:Organic-phase enzymology  Olive oils  Phenols  Tyrosinase
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