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Comparison of Chemometric and Chromatographic Methods to Obtain Kinetic Parameters for Textile Dyes during a Biodegradation Process
Abstract:The concentrations of three industrial-grade textile dyes were determined in a mixture after degradation by the fungus Ganoderma sp, by using the methods of UV-Vis spectrophotometry associated with Partial Least Squares regression and HPLC and comparing the results obtained from both methods. Using the concentrations calculated from the two methods, a kinetic study of the biodegradation mediated by the fungus was performed. The rate constants and the activation energies for this transformation were obtained for each dye in the mixture. The concentration of Remazol Blue R ESP could be determined by the HPLC method, and the value obtained was comparable with the result using the Partial Least Squares regression method. The Partial Least Squares regression method offers advantages over the HPLC method for the quantification of dyes in textile effluents, as it provides the kinetic parameters of the biodegradation reaction.
Keywords:Degradation  Dyes  Ganoderma sp  HPLC  PLS  Textile effluent
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