Chlorodesmethyldiazepam Fluorimetric Determination in Pharmaceutical Formulations and in Aqueous Media

Abstract

A direct fluorimetric method for the determination of chlorodesmethyldiazepam was proposed. The fluorescence process was pH dependent. The dynamic range for the method was 1.6–12 µg ml^–1. A linear relationship between the fluorescence intensity and the concentration of chlorodesmethyldiazepam solution was obtained with r ² of 0.9958. The detection limit for the method was found to be 0.224 µg ml^–1. The method has successfully applied to the determination of chlorodesmethyldiazepam in pure, authentic, and aqueous samples.